ISO INTERNATIONAL STANDARD 12871 Secondedition 2019-03 Olive oils and olive-pomace oils 一 Determination of aliphatic and triterpenic alcohols content by capillary gas chromatography Huiles d'olive et huiles de grignons d'olive - Détermination de la teneur en alcools aliphatiques et triterpeniques par chromatographie enphasegazeusesurcolonnecapillaire Referencenumber IS012871:2019(E) ISO @IS02019 IS0 12871:2019(E) COPYRIGHTPROTECTEDDOCUMENT IS02019 All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below orIso'smemberbody in the country of the requester. ISO copyright office CP 401: Ch. de Blandonnet 8 CH-1214 Vernier, Geneva Phone:+41227490111 Fax:+41227490947 Email:[email protected] Website: www.iso.org Published in Switzerland ii @IS0 2019-All rights reserved IS012871:2019(E) Contents Page Foreword .iv 1 Scope. ..1 2 Normativereferences ..1 3 Terms and definitions ..1 4 Principle .1 5 Reagents. ..1 6 Apparatus. .2 7 Sampling. .3 8 Preparation ofthetestsample .4 9 Procedure .4 9.1 Preparationoftheunsaponifiablematter .4 9.2 Separation of alcoholic fractions 4 9.3 Preparationofthetrimethylsilylethers .5 9.4 Gas chromatographic analysis.. .6 9.4.1 Preliminaryoperationsandcolumnpacking .6 9.4.2 Operatingconditions. 6 9.4.3 Analytical procedure .7 9.4.4 Peakidentification. 7 9.4.5 Quantitativeevaluation .7 10 Precision .7 10.1 Interlaboratorytest 7 10.2 Repeatability 8 10.3 Reproducibility .8 11 Test report. .8 AnnexA (informative)Thin-layerchromatographyseparationexampleand chromatogram examples 9 AnnexB(informative)Resultsofaninterlaboratorytest .12 Bibliography .13 IS02019-All rights reserved ili