ISO INTERNATIONAL STANDARD 14239 Secondedition 2017-07 Soil quality Laboratory incubation systems for measuring the mineralization of organic chemicals in soil under aerobic conditions Qualite du sol - Systemes d'incubation de laboratoire destinés a la mesure de la minéralisation de produits chimiques organiques dans le solenconditionsaérobies Reference number IS0 14239:2017(E) International Organization for Standardization @ IS0 2017 e=ZHEJIANG INST OF STANDARDIZATION C1 5956617 HS unde ted without license from IHS IS0 14239:2017(E) COPYRIGHT PROTECTED DOCUMENT IS0 2017, Published in Switzerland All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below or ISO's member body in the country of the requester. ISO copyright office Ch. de Blandonnet 8 . CP 401 CH-1214 Vernier, Geneva, Switzerland Tel. +41 22 749 01 11 Fax +41 22 749 09 47 [email protected] www.iso.org icensee-ZHEJIANG INST.OF STANDARDIZoISQ s31.7 - All rights reserved networking permited without license from IHS IS0 14239:2017(E) Contents Page Foreword ..iv Introduction. ..V 1 Scope. ..1 2 Normative references 3 Terms and definitions ..1 4 Methods ..1 4.1 General requirements .1 4.1.1 Soil collection and characterization .2 4.1.2 Test material. .2 4.1.3 Incubation conditions. .2 4.2 Choice of incubation systems. 2 4.3 Flow-through system. .4 4.3.1 Principle. .4 4.3.2 Materials and reagents ..5 4.3.3 Apparatus and glassware .6 4.3.4 Procedure. .6 4.4 Soda-lime column system .7 4.4.1 Principle .7 4.4.2 Materials and reagents ..8 4.4.3 Apparatus and glassware .9 4.4.4 Procedure. .9 4.5 Biometer system 12 4.5.1 Principle .12 4.5.2 Materials and reagents .13 4.5.3 Apparatus and glassware .14 4.5.4 Procedure .14 4.6 Radiorespirometer .14 4.6.1 Principle. ..14 4.6.2 Materials and reagents .15 4.6.3 Apparatus, glass- and plastic-ware .15 4.6.4 Procedure .15 4.7 Microradiorespirometer .16 4.7.1 Principle ..16 4.7.2 Materials and reagents .16 4.7.3 Apparatus, and plastic-ware. ..16 4.7.4 Procedure .17 4.8 Miniaturized respirometer .17 4.8.1 Principle ..17 4.8.2 Materials and reagents 4.8.3 Apparatus, and plastic-ware. ..18 4.8.4 Procedure. ..19 5 Calculation and expression of results ..19 5.1 For unlabelled test materials. .19 5.2 For 14C-labelled test materials. ..20 6 Test report ..20 Bibliography ..21 ii Licensee=ZHEJIANG INST OF STANDARDIZATION C1 5956617 ed without license from IHS