ISO INTERNATIONAL STANDARD 16014-5 Secondedition 2019-05 Plastics Determination of average molecular weight and molecular weight distribution of polymers using size-exclusion chromatography - Part 5: Light-scattering method Plastiques-Determination de la masse moleculairemoyenne et de la distribution des masses moleculaires de polymerespar chromatographie d'exclusionsterique- Partie 5:Methode pardiffusion lumineuse Referencenumber ISO16014-5:2019(E) ISO @ISO2019 IS016014-5:2019(E) COPYRIGHTPROTECTEDDOCUMENT @ISO2019 All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may be reproduced or utilized otherwise in anyform or byany means,electronic or mechanical, including photocopying,or posting on the internet or an intranet, without prior written permission. Permission can be requested from either IsO at the address beloworIso'smemberbodyinthecountryoftherequester ISO copyright office CP 401· Ch. de Blandonnet 8 CH-1214 Vernier, Geneva Phone:+41227490111 Fax:+4122749 09 47 Email: [email protected] Website:www.iso.org Published in Switzerland ii IS0 2019-All rights reserved IS016014-5:2019(E) Contents Page Foreword .. 1 Scope ..1 2 Normative references 3 Terms and definitions 4 Symbols. .2 5 Principle .2 5.1 SEC .2 5.2 Light-scattering SEC .2 6 Reagents ..3 7 Apparatus. .3 8 Procedure. .5 8.1 Preparationofcalibration solutions 5 8.2 Preparationofa solutionfordeterminingtheL-point 5 8.3 Preparation of sample solutions 5 8.4 Preparationof solutionsforcolumnperformanceevaluation 5 8.5 Setting up the apparatus 5 8.6 Operatingparameters. .5 8.6.1 Flow rate. 5 8.6.2 Injectionmassesandinjectionvolumes 5 8.6.3 Column temperature 6 8.6.4 Detector sensitivity 6 8.7 Numberofdeterminations. .6 9 Calibration .6 9.1 Calibration of concentration-sensitive detector and light-scattering detector .6 9.1.1 General. 6 9.1.2 Calibration method A .6 9.1.3 CalibrationmethodB .7 9.1.4 Calibrationmethod C 7 9.2 Determination of delay volume .8 9.3 Normalization ofdetector sensitivity 8 9.4 Determination ofrefractive index increment .8 10 Data acquisition and processing .8 10.1 Data acquisition 8 10.2 Evaluationofdataandcorrectionofchromatograms 8 10.3 Dataprocessing 8 10.3.1 Baselinedetermination .8 10.3.2 Determination of calculation range 9 10.3.3 Calculation of signal intensity .9 10.3.4 Calculation of molecular weight 9 10.3.5 Second virial coefficient, A2 9 11 Expression ofresults. ..10 11.1 Calibration curve. .10 11.1.1 General .10 11.1.2 MethodA .10 11.1.3 MethodB 10 11.2 Calculation ofaverage molecular weight ..12 11.3 Differentialmolecularweightdistributioncurve .12 11.4 Cumulative molecular weight distribution curve ..12 12 Precision ..13 IS0 2019 -All rights reserved iii