ISO INTERNATIONAL STANDARD 17556 Third edition 2019-05 Plastics Determination of the ultimate aerobic biodegradability of plastic materials in soil by measuring the oxygen demand in a respirometer or the amount of carbon dioxide evolved Plastiques - Détermination de la biodégradabilité aérobie ultime des matériaux plastiques dans le sol par mesure de la demande en oxygene dans un respirometre ou de la teneur en dioxyde de carbone libéré Reference number IS0 17556:2019(E) os1 @ IS0 2019 IS0 17556:2019(E) COPYRIGHTPROTECTEDDOCUMENT @ IS0 2019 All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested from either IsO at the address below or Iso's member body in the country of the requester. ISO copyright office CP 401 : Ch. de Blandonnet 8 CH-1214 Vernier, Geneva Phone: +41 22 749 01 11 Fax: +41 22 749 09 47 Email: [email protected] Website: www.iso.org Published in Switzerland ii @ IS0 2019 - All rights reserved IS0 17556:2019(E) Contents Page Foreword ..iv Introduction. ..V 1 Scope. ..1 2 Normative references 3 Terms and definitions ..1 4 Principle ..3 5 Test environment ..4 6 Materials. ..4 7 Apparatus. .4 8 Procedure 4 8.1 Preparation of the test material .4 8.2 Preparation of the reference material .5 8.3 Preparation of the test soil. .5 8.3.1 Collection and sieving of soil .5 8.3.2 Preparation of standard soil .6 8.3.3 Measurementofsoilcharacteristics .7 8.3.4 Adjustment of the water content and the pH of the soil .7 8.3.5 Handling and storage of the soil 7 8.4 Start-up and execution of the test 7 9 Calculation and expression of results. .9 9.1 Calculation .9 9.1.1 Percentage biodegradation from oxygen consumption values. 9 9.1.2 Percentage biodegradation from carbon dioxide evolved 9.2 Expression and interpretation of results ..10 10 Validity of results .10 11 Test report ..10 Annex A (informative) Principle of a manometric respirometer (example) ..12 Annex B (informative) Example of a system for measuring the amount of carbon dioxide evolved .13 Annex C (informative) Examples of methods for the determination of evolved carbon dioxide....14 Annex D (informative) Theoretical oxygen demand (ThoD) ..16 Annex E (informative) Example of a determination of the amount and the molecular mass of water-insoluble polymer remaining at the end of a biodegradation test ..17 Annex F (informative) Examples of long-term tests ..18 Annex G (informative) Interlaboratory test ..22 Bibliography .26 @ IS0 2019 - All rights reserved ii