ISO INTERNATIONAL STANDARD 21317 First edition 2019-02 Traditional Chinese medicine - Lonicera japonica flower Médecine traditionnelle chinoise - Fleur de Lonicera japonica Reference number IS0 21317:2019(E) osi @IS02019 IS0 21317:2019(E) COPYRIGHTPROTECTEDDOCUMENT @ IS0 2019 All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested from either IsO at the address below or Iso's member body in the country of the requester. ISO copyright office CP 401 : Ch. de Blandonnet 8 CH-1214 Vernier, Geneva Phone: +41 22 749 01 11 Fax: +41 22 749 09 47 Email: [email protected] Website: www.iso.org Published in Switzerland ii @ IS0 2019 - All rights reserved IS0 21317:2019(E) Contents Page Foreword ..iv Introduction. .. 1 Scope. ..1 2 Normative references 3 Terms and definitions 4 Descriptions ..2 5 Requirements .4 5.1 General characteristics 4 5.2 Macroscopic characteristics .4 5.3 Microscopic characteristics .4 5.4 Moisture .5 5.5 Total ash .5 5.6 Acid-insoluble ash .5 5.7 Dilute ethanol-soluble extract 5 5.8 Identification of chlorogenic acid and luteoloside .5 5.9 Chlorogenic acid content .6 5.10 Luteoloside content ..6 5.11 Heavy metals content .6 6 Sampling. ..6 7 Test methods. .7 7.1 Macroscopic identification ..7 7.2 Microscopic identification .7 7.3 Determination of moisture content .7 7.4 Determination of total ash content .7 7.5 Determination of acid-insoluble ash content .7 7.6 Determination of dilute ethanol-soluble extract content 7 7.7 Identification of chlorogenic acid and luteoloside. 7 7.8 Determinationofchlorogenicacidcontent .7 7.9 Determinationofluteolosidecontent. 7.10 Determination of heavy metals content. 7 8 Test report. ..8 9 Packaging, storage and transportation ..8. 10 Marking and labelling Annex A (informative) Identification of chlorogenic acid and luteoloside ..9 Annex B (informative) Determination of chlorogenic acid content ..11 Annex C (informative) Determination of luteoloside content. ..13 Annex D (informative) Reference values of national and regional limits of moisture, total ash, acid-insoluble ash, dilute ethanol-soluble extract, chlorogenic acid, luteoloside and heavy metals in Lonicera japonica flower.. ..16 Bibliography ..18 @ IS0 2019 - All rights reserved ii