ISO INTERNATIONAL STANDARD 22212 First edition 2019-03 Traditional Chinese medicine - Gastrodia elata tuber Médecine traditionnelle chinoise - Tubercule de Gastrodia elata Reference number IS0 22212:2019(E) ISO @IS02019 IS0 22212:2019(E) COPYRIGHTPROTECTEDDOCUMENT @ ISO 2019 All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested from either IsO at the address below or Iso's member body in the country of the requester. ISO copyright office CP 401 : Ch. de Blandonnet 8 CH-1214 Vernier, Geneva Phone: +4122 749 0111 Fax: +41 22 749 09 47 Email: [email protected] Website: www.iso.org Published in Switzerland ii @ IS0 2019 - All rights reserved IS0 22212:2019(E) Contents Page Foreword ..iv Introduction. ..V 1 Scope. .1 2 Normative references ..1 3 Terms and definitions ..1 4 Descriptions ..2 5 Requirements .4 5.1 General characteristics .4 5.2 Morphological features ..4 5.3 Moisture .4 5.4 Total ash ..4 5.5 Dilute ethanol-soluble extract .4 5.6 Identification of marker compound(s) .4 5.7 Content of marker compound(s) 4 5.8 Heavymetals .4 5.9 Pesticide residues .5 5.10 Sulfur dioxide ..5 6 Sampling. ..5 7 Test methods. .5 7.1 Macroscopic identification .5 7.2 Determination of moisture content .5 7.3 Determination of total ash content 7.4 Determination of dilute ethanol-soluble extract content .6 7.5 Identification of marker compound(s) .6 7.6 Determination of marker compound(s) content ..6 7.7 Determination of heavy metals. 7.8 Determination of pesticide residues .6 7.9 Determination of sulfur dioxide content 8 Test report ..6 9 Packaging, storage and transportation 10 Marking and labelling ..7 Annex A (informative) Determination of moisture content ..8 Annex B (informative) Determination of dilute ethanol-soluble extract content ..9 Annex C (informative) Identification of gastrodin ..10 Annex D (informative) Identification of p-hydroxybenzyl alcohol. ..12 Annex E (informative) Determination of gastrodin and p-hydroxybenzyl alcohol contents .14 Annex F (informative) Reference values of national and regional limits in Gastrodia elata tuber..17 Bibliography .18 @ IS0 2019 - All rights reserved iii