ISO INTERNATIONAL STANDARD 22253 First edition 2019-04 Cigarettes Determination of nicotine in total particulate matter from the mainstream smoke with an intense smoking regime - Gas- chromatographic method Cigarettes - Dosage de la nicotine dans la matiere particulaire totale Reference number IS0 22253:2019(E) rso @IS02019 IS0 22253:2019(E) COPYRIGHTPROTECTEDDOCUMENT @ IS0 2019 All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested from either IsO at the address below or Iso's member body in the country of the requester. ISO copyright office CP 401 : Ch. de Blandonnet 8 CH-1214 Vernier, Geneva Phone: +41 22 749 01 11 Fax: +41 22 749 09 47 Email: [email protected] Website: www.iso.org Published in Switzerland ii @ IS0 2019 - All rights reserved IS0 22253:2019(E) Contents Page Foreword .iv Introduction. ..V 1 Scope. ..1 2 Normative references 3 Terms and definitions 4 Principle ..1 5 Reagents. ..1 6 Apparatus ..2 7 Procedure .2 7.1 Testportion .2 7.2 Setting up the apparatus .3 7.3 Calibration of the gas chromatograph .3 7.4 Determination .3 8 Expression of results 3 9 Repeatability and reproducibility ..4 10 Alternative gas-chromatographic procedures and analysis precautions ..4 10.1 General ..4 10.2 Alternative columns. .5 10.2.1Packed columns. .5 10.2.2 Capillary columns .5 10.3 Injection systems .5 10.4 Alternative internal standards .5 11 Test report ..5 Annex A (informative) Example of chromatogram .6 Bibliography 7 @ IS0 2019 - All rights reserved iii