ISO INTERNATIONAL STANDARD 13629-2 First edition 2014-06-15 Textiles Determination of antifungal activity of textile products Part 2: Plate count method Textiles - Détermination de I'activite antifongique des produits textiles- Partie 2: Methode par dénombrement sur plaque de gelose Reference number ISO 13629-2:2014(E) LSO @ IS0 2014 HS under ted without license from IHS Not for Resale IS0 13629-2:2014(E) COPYRIGHTPROTECTEDDOCUMENT @ IS0 2014 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below or ISO's member body in the country of the requester. ISO copyright office Case postale 56:CH-1211 Geneva 20 Tel. + 41 22 749 01 11 Fax + 41 22 749 09 47 E-mail [email protected] Web www.iso.org Published in Switzerland @ IS0 2014 - All rights reserved No reproductic networking permited without license from IHS Not for Resale IS0 13629-2:2014(E) Contents Page Foreword ..iv Introduction. ..V 1 Scope. .1 2 Normative reference 3 Terms and definitions ..1 4 Principle ..2 5 Safety precaution .2 6 Reference fungi. ..2 7 Apparatus ..2 8 Reagents and culture media .4 8.1 Purewater ..4 8.2 Anionic surfactant 4 8.3 Culture medium ..4 9 Fungi preservation and use .6 10 Spore suspension .6 10.1 General .6 10.2 Suspending spores in culture media .7 10.3 Collection and dispersion of spore suspension from a culture medium .7 10.4 Filtering to remove hyphae and spore thread. .7 10.5 Using centrifuge and re-suspension to remove supernatant. .7 10.6 Confirming the concentration of spore suspension .8 10.7 Adjusting spore suspension for testing ..8 10.8 Enumeration of inoculum. ..8 11 Testing procedure ..8 11.1 Inoculation and preparation of specimens 11.2 Plate count method procedure .11 12 Test results. ..13 12.1 Judgment of test effectiveness ..13 12.2 Calculation of antifungal activity value ..13 13 Test report ..14 Annex A (normative)Fungi used in this part of Is0 13629 ..15 Annex B (informative) Antifungal efficacy ..16 Bibliography ..17 iii Stal se with ISt thout license from IHS Not for Resale