ISO INTERNATIONAL STANDARD 13859 First edition 2014-02-01 Soil quality - Determination of polycyclic aromatic hydrocarbons (PAH) by gas chromatography (GC) and high performance liquid chromatography (HPLC) Qualite du sol-Determination des hydrocarbures aromatiques polycycliques (HAP) par chromatographie en phase gazeuse (CPG) et chromatographie liquide a haute performance (CLHP) Reference number IS013859:2014(E) LSO @IS02014 icense from IHS Not for Resale IS013859:2014(E) COPYRIGHTPROTECTEDDOCUMENT @ISO2014 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below or ISO's member body in the country of the requester. ISO copyright office Case postale 56.CH-1211 Geneva 20 Tel. + 4122 749 0111 Fax +41 22 749 09 47 E-mail [email protected] Web www.iso.org Published in Switzerland @ IS0 2014 - All rights reserved No reproduction or networking permited without license from IHS Not for Resale IS0 13859:2014(E) Contents Page Foreword ..iv Introduction.. 1 Scope.. 2 Normative references 3 Terms and definitions ..2 4 Principle 5 Interferences .3 5.1 Interference with sampling and extraction .3 5.2 Interference with GC-MS 5.3 InterferenceswiththeHPLC .4 6 Safety remarks. .4 7 Reagents .5 7.1 General .5 7.2 Reagents for extraction. .5 7.3 Reagents for clean-up.. .5 7.4 Reagents for chromatographic analysis .6 7.5 .1 Standards. 7.6 Preparation of standard solutions .8 7.7 Preparation of internal standard solutions ..9 7.8 Preparation of injection standard solution .9 8 ..9 Apparatus 9 Sample storage and preservation .11 9.1 Sample storage. .11 9.2 Samplepretreatment ..11 10 Procedure .11 10.1 Blanktest .11 10.2 Extraction. .12 10.3 Concentrationordilution ..14 10.4 Clean-up of the extract .15 10.5 Addition of the injection standard .16 10.6 Gas chromatographic analysis (GC) .17 10.7 High performance liquid chromatographic analysis (HPLC) 20 11 Performance characteristics .23 12 Precision .23 13 Test report ..23 AnnexA(informative)Repeatabilityandreproducibilitydata .24 AnnexB (informative)Examplesof instrumental conditionsand chromatograms ..27 Bibliography ..37 iii No reproduction or networking permi ed without license from IHS Not for Resale