ISO INTERNATIONAL STANDARD 12782-5 First edition 2012-06-01 Soil quality Parameters for geochemical modelling of leaching and speciation of constituents in soils and materials Part 5: Extraction of humic substances from aqueous samples Qualite du solParametres pourla modelisation géochimique de la lixiviation et de la spéciation des constituants des sols et des materiaux Partie 5: Extraction des substances humiques des échantillons aqueux Reference number ISO 12782-5:2012(E) ISO CopyrightInternational Organization for Standardization @ISO2012 ted without license from IHS Not for Resale ISO12782-5:2012(E) COPYRiGHTPROTECTEDDOCUMENT ISO 2012 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying and microfilm, without permission in writing from either ISO at the address below or ISO's member body in the country of the requester. ISO copyright office Case postale 56 · CH-1211 Geneva 20 Tel. + 41 22 749 01 11 Fax + 41 22 749 09 47 E-mail [email protected] Web www.iso.org Published in Switzerland ISO 2012-All rights reserved or networking permited withoutlicense from IHS Not for Resale ISO12782-5:2012(E) Contents Page Foreword ..iv Introduction 1 Scope 2 Normative references 3 Terms and definitions 4 Principle 5 Apparatus . 3 6 Reagents.. 3 7 Sampling. 7.1 Laboratory sample 7.2 Test sample. 7.3 Test portion 8 Procedure 8.1 Preparation of DAX-8 resin.. 8.2 Determination of total humic acid (HA), fulvic acid (FA) and hydrophilic organic carbon (Hy) content in aqueous samples. 5 9 Eluate treatment and storage. 10 Analytical determination ..5 11 Blanktest 5 12 Calculation. 12.1 General correction factors for the calculation of humic acid (HA), fulvic acid (FA), hydrophilic organic carbon (Hy) and hydrophobic neutral organic carbon (HoN) in aqueous samples.... .6 12.2 Concentration of total humic acid (HA), fulvic acid (FA), hydrophilic organic carbon (Hy) and hydrophobic neutral organic carbon (HoN) concentrations in aqueous samples ... .7 13 Expression of results 8 14 Test report .8 15 Performance characteristics Annex A (informative) Schematic representation of the fractionation procedure .9 Annex B (informative) Validation of procedure 11 Annex C (informative) Conditions regarding centrifugation. Bibliography.. ili ed without license from IHS Not for Resale