ISO INTERNATIONAL STANDARD 17512-1 First edition 2008-12-15 Soil quality Avoidance test for determining the quality of soils and effects of chemicals on behaviour. Part 1: Test with earthworms (Eisenia fetida and Eisenia andrei) Qualite du solEssaid'évitement pour controlerla qualitedes sols et les effets des produits chimiques sur le comportement- Partie 1: Essai avec des vers de terre (Eisenia fetida et Eisenia andrei) Reference number ISO 17512-1:2008(E) @ ISO 2008 Not for Resale ISO 17512-1:2008(E) PDF disclaimer This PDF file may contain embedded typefaces. In accordance with Adobe's licensing policy, this file may be printed or viewed but shall not be edited unless the typefaces which are embedded are licensed to and installed on the computer performing the editing. In accepts no liability in this area. Adobe is a trademark of Adobe Systems Incorporated. Details of the software products used to create this PDF file can be found in the General Info relative to the file; the PDF-creation parameters were optimized for printing. Every care has been taken to ensure that the file is suitable for use by IsO member bodies. In the unlikely event that a problem relating to it is found, please inform the Central Secretariat at the address given below. COPYRIGHT PROTECTED DOCUMENT ISO2008 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying and microfilm, without permission in writing from either isO at the address below or ISO's member body in the country of the requester. ISO copyright office Case postale 56 . CH-1211 Geneva 20 Tel. + 4122 749 01 11 Fax + 41 22 749 09 47 E-mail [email protected] Web www.iso.org Published in Switzerland @ ISO 2008 - All rights reserved I without license from IHS Not for Resale ISO 17512-1:2008(E) Contents Page Foreword Introduction 1 Scope 2 Normative references 3 Terms and definitions 4 Principle ... 5 Reagents and materials. 6 Apparatus.. 7 Procedure... 7.1 Appropriate concentration range 7.2 Testing of soils.. 7.3 Testing of chemical.... 7.4 Reference substance 7.5 Validity criteria.... 8 Calculation and expression of results .. 6 9 Testreport. Annex A (informative) Test chambers .8 Annex B (informative) Example of a breeding technique for Eisenia fetida and Eisenia andrei.... Annex C (informative) Further test organisms.. Annex D (informative) Contaminants that earthworms can detect and avoid in the avoidance test.....13 Annex E (normative) Testing of chemicals in the avoidance test.. .14 Annex F (normative) Determination of water-holding capacity .. .16 Annex G (informative) Comparison of the results obtained in the two section chamber and six sectionchambersystem..... Annex H (informative) Influence of soil properties on avoidance behaviour Basis for the threshold value of 20 %..... Annex I (informative) Data gained in “dual" tests with the same (untreated) control soil on both sidesofthetestvessels.... 22 Bibliography. 24 ili from IHS Not for Resi