ISO INTERNATIONAL STANDARD 7346-1 Second edition 1996-06-15 Water quality Determination of the acute lethal toxicity of substances to a freshwater fish [Brachydanio rerio Hamilton-Buchanan (Teleostei, Cyprinidae)J -- Part 1: Static method Qualite de I'eau - Determination de la toxicite aigue letale de substances vis-a-vis d'un poisson d'eau douce [Brachydanio rerio Hamilton-Buchanan (Téléostei, Cyprinidae) Partie 1: Méthode statique This material is reproduced from Iso documents under Internationai Organization for Standardization (Iso) Copyright License number IHS/Icc/1996. Not for resale. No part of these iso documents may be reproduced in any form, electronic retrieval system or otherwise, except as aliowed in the copyright law of the country of use, or with the prior written consent of Iso (Case postale 56, 1211 Geneva 20, Switzerland, Fax +41 22 734 10 79), IHs or the iso Licensor's members. ISO Reference number ISO 7346-1:1996(E) Copyright International Organization for Standardizatior No reproduction or networking permitted without license from IHS Not for Resale ISO 7346-1:1996(E) Foreword IsO (the International Organization for Standardization) is a worldwide federation of national standards bodies (IsO member bodies). The work technical committees.E Each member body interested in a subject for represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. IsO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote ISO/TC147,Water quality.Subcommittee SC 5.Biological methods. Thissecond editioncancelsandreplacesthe first edition (ISO 7346-1:1984), which has been technically revised. ISO 7346 consists of the following parts, under the general title Water quality 一 L Determination of the acute lethal toxicity of substances to a freshwater fish [Brachydanio rerio Hamilton-Buchanan (Teleostei, Cyprinidae]: Part1:Staticmethod Part 2: Semi-static method - Part 3: Flow-through method Annexes A, B and C of this part of ISO 7346 are for information only. ISO1996 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced microfilm, without permission in writing from the publisher. International Organization for Standardization Case Postale 56 : CH-1211 Geneve 20 : Switzerland Printed in Switzerland ii onal Organization for Standardization Not for Resale CISO IS07346-1:1996(E) Introduction The three parts of iSO 7346 describe methods of determining the acute Hamilton-Buchanan) but it must be emphasized that the recommended use of the zebra fish does not preclude the use of other species. The methodologies presented here may also be used for other species of freshwater, marine or brackish water fish, with appropriate modifications of, for example, dilution water quality and the temperature conditions of the test. semi-static and flow-through methods. The static test, described in this of requiring simple apparatus, although the substances in the test vessel may become depleted during the course of the test and the general quality of the water may deteriorate. The flow-through method, described in comes such problems but requires the use of more complex apparatus. In the semi-static procedure, described in ISO 7346-2, the test solutions are renewed every 24 h or 48 h, this method being a compromise be- tween the othertwo. The flow-through method can be used for most types of substances, in- cluding those unstable in water, but the concentrations of the test sub- stance are determined wherever possible. The static method is limited to the study of substances whose tested concentrations remain relatively constant during the test period. The semi-static method can be used for highlyvolatile