ISO INTERNATIONAL STANDARD 875 Second edition 1999-09-15 Essential oils Evaluation of miscibility in ethanol Huilesessentielles Evaluation de la miscibilité a I'éthanol ISO Reference number ISO 875:1999(E) T by IHS under lic itted without license from IHS Not for Resale ISO 875:1999(E) Foreword IsO (the International Organization for Standardization) is a worldwide federation of national standards bodies (isO memberbodies).TheworkofpreparingInternationalStandardsisnormallycarried out throughISOtechnical liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote International Standard ISO 875 was prepared by Technical Committee ISO/TC 54, Essential oils. @ISO1999 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, electronic InternationalOrganizationforStandardization Casepostale56·CH-1211Geneve20·Switzerland Internet [email protected] Printed in Switzerland Not for Resale ISO 875:1999(E) INTERNATIONAL STANDARD @ISO Essential oils Evaluation of miscibility in ethanol 1 Scope ethanol and water of known ethanol content. 2 Normative references The following normative documents contain provisions which, through reference in this text, constitute provisions of thisInternational Standard.For dated references,subsequent amendments to,or revisions of,any of these maintain registers of currently valid International Standards. IsO 356, Essential oils Preparation of test sample. IsO 648, Laboratory glassware -One-mark pipettes. 3 Principle Evaluation of miscibility and possibly of opalescence. 4 Classification of miscibility remains so after further gradual addition of ethanol of the same concentration up to a total of 20 volumes. 4.2 An essential oil is said to be miscible with V volumes of ethanol of a given concentration, at a temperature of 20 C, and to become cloudy when diluted in V'volumes, when the mixture of 1 volume of the oil in question with V volumes of the ethano! is clear and becomes cloudy after further gradual addition of (V'- V) volumes of ethano! of the same concentration and remains cloudy after further addition of the ethanol up to a total of 20 volumes. 4.3 An essential oil is said to be miscible with V volumes of ethanol of a given concentration, at a temperature of with V volumes of the ethanol is clear, becomes cloudy after further gradual addition of (v'- V) volumes of ethanol same concentration. concentration(undertheconditionsasgivenin4.1,4.2and4.3)showsanopalescenceidenticalwiththeoneofthe standard solution for opalescence, freshly prepared in accordance with the method given in 5.3. NOTE The numerical values of V, V'and v" are not more than 20. CopyrightIntenational Organizationfor Standardization 1 Not or Resale