ISO INTERNATIONAL STANDARD 9439 Second edition 1999-03-01 Water quality Evaluation of ultimate aerobic biodegradability of organic compounds in aqueous medium Carbon dioxide evolution test Qualité de I'eau- Evaluation de la biodégradabilité aerobie ultime en milieu aqueux des composés organiques Essai de dégagement de dioxyde de carbone ISO Referencenumber ISO 9439:1999(E) No r y IHS under ted without license from IHS Not for Resale ISO 9439:1999(E) Contents 1 Scope 2 Definitions 3 Principle 4 Test environment.. 3 5 Reagents 3 6Apparatus 7Procedure.. 5 8Calculation 9 Validity of results. 10 Test report . Annex A (informative) Principle of a test system for measuring carbon dioxide (example) .. 10 AnnexB(informative)Examplesofthedeterminationofreleasedcarbondioxide.. Annex C (informative) Example of a biodegradation curve, .13 Annex D (informative) Combined determination of carbon dioxide and DoC. 14 Bibliography.... ISO1999 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying and microfilm, without permission in writing from the publisher. InternationalOrganizationforStandardization Case postale 56 . CH-1211 Geneve 20 · Switzerland Internet [email protected] Printed in Switzerland ational Organization for Standardizatior Not for Resal @ISO ISO9439:1999(E) Foreword ISO(theInternationalOrganizationforStandardization)isaworldwidefederationofnationalstandardsbodies(lSo member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has liaison with IsO, also take part in the work. iSO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization International Standards are drafted in accordance with the rules given in the ISO/lEC Directives, Part 3. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. International Standard ISO 9439 has been prepared by Technical Committee ISO/TC 147, Water quality, Subcommittee SC 5, Biological methods. Thissecondeditioncancelsandreplacesthefirstedition(lsO9439:199o),whichhasbeentechnicallyrevised ii