ISO INTERNATIONAL STANDARD 8294 First edition 1994-11-01 Animal and vegetable fats and oils -- Determination of copper, iron and nickel contents -- Graphite furnace atomic absorption method Corps gras d'origines animale et vegetale - Determination de la teneur atomiqueavec fourengraphite ISO Reference number ISO 8294:1994(E) aiganzatSandadizai No reproduction or networking permitted without license from IHS Not for Resale ISO 8294:1994(E) Foreword Iso (the International Organization for Standardization) is a worldwide federation of national standards bodies (isO member bodies). The work of preparing International Standards is normally carried out through isO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with IsO, also take part in the work. IsO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. International Standard ISO 8294 was prepared by Technical Committee ISO/TC 34, Agricultural food products, Subcommittee SC 11, Animal and vegetable fats and oils. Annex A of this International Standard is for information only. ISO 1994 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, electronic or mechanicai, including photocopying and microfilm, without permission in writing from the publisher. International Organization for Standardization CasePostale56·CH-1211Geneve20·Switzerland Printed in Switzerland Copyiah Intenatinal Oorganizatin for Standardization No reproduction or networking permitted without license from IHS Not for Resale INTERNATIONAL STANDARD ISO ISO 8294:1994(E) Animal and vegetable fats and oils Determination of copper, iron and nickel contents - Graphite furnace atomic absorption method Scope 4 Reagents This International Standard specifies a method for the Use only reagents of recognized analytical grade and determination of trace amounts of copper, iron and water in accordance with grade 2 of IsO 3696. nickel in animal and vegetable fats and oils, referred to hereinafter as fats. 4.1 Aluminium oxide, analytical grade. 2 Normative references NOTE 1 A suitable aluminium oxide (Quality No. 1077) is available from Merck, D-1600 Darmstadt, Germany.1] The following standards contain provisions which, through reference in this text, constitute provisions 4.2 Dilution oil, for example a refined edible oil, of this International Standard. At the time of publi- liquid at ambient temperature. cation, the editions indicated were valid. All standards are subject to revision, and parties to agreements Store the oil in a metal-free polyethylene or based on this International Standard are encouraged polyethylene bottle (5.1). Metal contents of the oil to investigate the possibility of applying the most re- shall be not greater than: cent editions of the standards indicated below. Members of IEC and ISO maintain registers of cur- Cu, 3 μg/kg; rently valid International Standards. Fe, 5 μg/kg; and IsO 661:1989, Animal and vegetable fats and oils Preparation of test sample. Ni, 5 μg/kg. Is03696:1987,Water for analytical laboratoryuse NOTE 2 A sample of near-metal-free oil may be obtained Specification and test methods. by the following procedure. Dissolve the oil in light petroleum (boiling range 40°C to 60 C) in a ratio of 1 kg of oii to 3 itres of ignt petroieum. 3 Principle Prepare an aluminium oxide column (diameter/height ratio 1/10) using twice the mass of aluminium oxide (activated Vaporization of a test portion of the fat in a graphite by heat