ISO INTERNATIONAL STANDARD 10260 Firstedition 1992-07-15 Water quality - Measurement of biochemical parameters - Spectrometric determination of the chlorophyll-a concentration Qualite de I'eau - Mesurage des parametres biochimiques -- Dosage spectrometriquedelachlorophyllea Reference number ISO10260:1992(E) Copyright International Organization for Standardization by IHS No reproduction or networking permitted without license from IHS Not for Resale ISO10260:1992(E) Foreword Iso (the International Organization for Standardization) is a worldwide federation of national standards bodies (isO member bodies). The work of preparing International Standards is normally carried out through Iso technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, govern- work. Iso collaborates closely with the International Electrotechnical Commission(lEC)onallmattersofelectrotechnicalstandardization. Draft International Standards adopted by the technical committees are national Standard requires approval by at least 75 % of the member bodies casting a vote. International Standard Iso 10260 was prepared by Technical Committee ISO/TC 147, Water quality. Sub-Committee SC 2, Physical, chemical, biochemical methods. C ISO 1992 All rights reserved. No part of this publication may be reproduced or utilized in any form permission in writing from the publisher. InternationatOrganizationforStandardization Case Postale 56.CH-1211 Geneve 20·Switzerland Printed in Switzerland ii CyrhnatiaganizatiStandardizatio No reproduction or networking permitted without license from IHS Not for Resale ISO10260:1992(E) Introduction Chlorophyll-a is the essential photosynthetic pigment present in all green plants. The chlorophyll content of a surface water is an indicator of its trophic state. The determination of the chlorophyll-a concentration provides information concerning the quantity and potential photosynthetic activity of algae. The most important metabolites of chlorophylls are phaeophytines and phaeophorbide. The,ratio of chlorophyll to phaeopigments is indicative of the physiological state of the algae. ili CopyrightinternatinalOrganizationfor Standardization No reproductior networking permitted without license from IHS Not for Resale