7609 International Standard OS1) ANS Essential oils - Analysis by.gas chromatography on capillary columns - General method Huiles essentielles -- Analyse par chromatographie en phase gazeuse sur colonne capillaire -- Methode generale First edition - 1985-12-01 UDC 665.5:543.544 Ref.No.ISO7609-1985 (E) Descriptors essentialoils,chemicalanalysis, chromatographicanalysis,gaschromatography Price based on 7 pages Copyright International Organizationfor Standardization Vot for Resale Foreword Iso (the International Organization for Standardization) is a worldwide federation of national standards bodies (isO member bodies). The work of preparing International Standards is normally carried out through Iso technical committees. Each member :body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, govern- imental and non-governmental, in liaison with so, also take part in the work. Draft International Standards adopted by the technical committees are circulated to thememberbodiesforapprovalbeforetheiracceptanceasInternationalStandardsby the iso Council. They are approved in accordance with iso procedures requiring at least 75 % approval by the member bodies voting. International Standard ISO 7609 was prepared by Technical Committee ISO/TC 54, Essentialoils. Usersshouldnotethat all InternationalStandards undergo revisionfromtimetotime and that any reference made herein to any other International Standard implies its latest edition, unless otherwise stated. International Organization for Standardization, 1985 Printed in Switzerland Copyright International Organization for Standardization No reproduction or networking permited without license from IHS Vot for Resale INTERNATIONAL STANDARD ISO7609-1985 (E) Essential oils -- Analysis by gas chromatography on capillary columns - General method 0Introduction If required, identification of the different constituents from their retention indexes. Since the description of methods of analysis by gas chromatography is very long, it is considered useful to establish Quantitative determination of specific constituents by measure- general methods ontheonehand,givingdetailed information ment of peak areas. on all the recurrent parameters, apparatus, products, methods, formulae,etc.,and onthe other hand standards with short details on the determination of specific constituents in the 4 Reagents and products the pertinent determination. During the analysis, unless otherwise specified, use only reagents of recognized analytical grade and freshly distlled These short-version standards will either refer to the present products. International Standard for gas chromatographic analyses on capillary columns or to ISo 7359 for analyses on packed 4.1 Carrier gas: hydrogen2), helium or nitrogen, according columns. to the type of detector used. If detectors are used which require carrier gases other than those mentioned, the carrier gas shall be specified. Scope and field of application 4.1.1 Auxiliary gases: any gases suitable for the detector This International Standard specifies a general method for the used.For a flame ionizationdetector air and hydrogen of high analysis of essential oilsbygas chromatographyon capillary purity. columns forthe purpose of determining the content of a specific constituent and/or searching for a characteristic 4.2 Product for checking the chemical inertness of the profile. column:linalylacetate,ofpurityat least98%. 4.3 Products for checking the efficiency of the 2 References column.3) So 356, Essential oils - 4.3.1 Linalol, of purity at least 99 % determined by Preparationoftestsample. chromatography. ISo7359,Essentialoils- -Analysis bygas chromatography on packed columns -- General method. 4.3.2 Methane, of purity at least 99 % determined by chromatography. 3Principle 4.4 Reference substance, corresponding to the con- stituent to be determined or detected. The reference substan