(ISo International Standard 7712 INTERNATIONAL ORGANIZATION FOR STANDARDIZATION+MEXKAYHAPOAHAH OPIAHM3AUWH HO CTAHAAPTW3AWW-ORGANISATION INTERNATIONALE DE NORMALISATION Laboratory glassware - Disposable Pasteur pipettes Verrerie delaboratoire-Pipettes Pasteura usage unique First edition - 1983-12-15 UDC 542.3 : 531.732 Ref.No.ISO7712-1983 (E) ISO 7712-1983 ( Descriptors : glassware, laboratory glassware, pipettes, dimensions, dimensional tolerances, specifications, designation, packages, marking. Price based on 2 pages No reproduction or networking permitted without license from IHS Not for Resale Foreword Iso (the International Organization for Standardization) is a worldwide federation of national standards bodies (isO member bodies). The work of developing International Standards is carried out through ISo technical committees. Every member body interested in a subject for which a technical committee has been authorized has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with Iso, also take part in the work. Draft International Standards adopted by the technical committees are circulated to the member bodies for approval before their acceptance as International Standards by the ISo Council. International Standard ISo 7712 was developed by Technical Committee ISo/TC 48, Laboratory glassware and related apparatus, and was circulated to the member bodies inJuly1982. It has been approved by the member bodies of the following countries : Australia India Romania Brazil Italy South Africa, Rep. of Czechoslovakia Korea, Rep. of United Kingdom France Netherlands USA Germany, F.R. Poland USSR Hungary Portugal No member body expressed disapproval of the document. @ International Organization for Standardization, 1983 Printed in Switzerland Copyright International Organization for Standardization Not for Resale INTERNATIONAL STANDARD ISO T712-1983 (E) Laboratory glassware - Disposable Pasteur pipettes Scope and field of application Designation This International Standard specifies requirements for the most The pipettes specified shall be designated by a number commonly used sizes of glass disposable Pasteur pipettes. representing the nominal overall length of the pipette, ex- pressed in millimetres,i.e.sizes145and230. The pipettes are uncalibrated and are intended for the transfer andreplicatedispensingofdropsofsolutions,andsuspensions of biological materials. 6 Construction 6.1 General 2 Reference The pipette shall be of sufficient strength and uniformity of wall Iso 8417, Laboratory volumetric instruments - Principles of thickness to withstand normal usage and transport when design and construction of disposable volumetric articles. 1) packed as specified in 8.1 and 8.2. 6.2 Material Definition The pipettes shall be made of glass of suitable chemical and disposable Pasteur pipettes : Pasteur pipettes intended to be thermalproperties.Theyshall beasfreeaspossiblefromvisible used once only and then discarded. defects,andshallbe reasonablyfreefrominternal stress 6.3 Resistance to sterilization 4 Sizes Thepipetteshallbesuitablefor,andnotadverselyaffectedby, 4.1 General one of the following methods : a) exposure to dry heat for (60 ± 2) min at a temperature Two sizes of pipette are specified, details of which are given in of (160±1) oC; the table, and the general form of the pipette is illustrated in the figure. b) exposure to saturated steam, in an autoclave, either for at least 20 min at (121 ± 1) oC or for at least 3,5 min at 4.2 Dimensions and tolerances (134 ± 1) °C. Nominal dimensions are specified in the table and manufac- 6.4 Top of pipette turers shall select values within the specified range. The dimen- sionsofpipettessupplied inanyonebatchshalfnotdifferfrom The top of the pipette shall be finished square with the axis of the nominal value for the batch by more than. the following the pipette, and shall be fire-polished. tolerances : 6.5 Delivery jet length