ISO INTERNATIONAL STANDARD 22718 Second edition 2015-12-01 Cosmetics Microbiology - Detection of Staphylococcus aureus Cosmétiques - Microbiologie - Détection de Staphylococcus aureus Reference number ISO 22718:2015(E) SO International Organization for Standardization ZHEJIANG INST OF STANDARDIZATION C15956617 IS02015 vided by IHS under itted without license from IHS IS0 22718:2015(E) COPYRIGHTPROTECTEDDOCUMENT IS0 2015, Published in Switzerland All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below or ISO's member body in the country of the requester. ISO copyrightoffice Ch. de Blandonnet 8 . CP 401 CH-1214 Vernier, Geneva, Switzerland Tel. +41 22 749 01 11 Fax +41 22 749 09 47 [email protected] www.iso.org PrganizationforStandardization icensee-ZHEJIANG INSTOF STANDARDIZAoISQ05 - All rights reserved Not for Resale, 2016/3/29 05:26:09 etworking permitted without license from IHS IS0 22718:2015(E) Contents Page Foreword ..iv Introduction. ..V 1 Scope. .1 2 Normative references 3 Terms and definitions .1 4 Principle .2 5 Diluents and culture media .2 5.1 General .2 5.2 Diluent for the bacterial suspension (tryptone sodium chloride solution) .3 5.2.1 General .3 5.2.2 Composition .3 5.2.3 Preparation. .3 5.3 Culture media. .3 5.3.1 General ..3 5.3.2 Agar medium for the suitability test (see Clause 11) [soybean-casein digest agar medium (SCDA) or tryptic soy agar (TSA)) ..3 5.3.3 Enrichment broth 5.3.4 Selective agar medium for isolation of Staphylococcus aureus ..5 6 Apparatus and glassware .6 7 .6 Strains of microorganisms 8 .6 Handling of cosmetic products and laboratory samples 9 Procedure .7 9.1 General recommendation 9.2 Preparation of the initial suspension in the enrichment broth 7 9.2.1 General .7 9.2.2 Water-miscible products 7 9.2.3 Water-immiscible products 9.2.4 Filterable products. .7 9.3 Incubation of the inoculated enrichment broth .7 9.4 Detection and identification of Staphylococcus aureus .7 9.4.1 Isolation .7 9.4.2 Identification of Staphylococcus aureus ..8 10 Expression of the results (detection of Staphylococcus aureus) ..8 11 Neutralization of the antimicrobial properties of the product .9 11.1 General .9 11.2 Preparation of inoculum. .9 11.3 Suitability of the detection method 9 11.3.1 Procedure. .9 11.3.2 Interpretation of suitability test results .9 12 Test report ..10 Annex A (informative) Other media ..11 Annex B (informative) Neutralizers of antimicrobial activity of preservatives and rinsing liquids .14 Bibliography ..15 iii ee=ZHEJIANG INST OF STANDARDIZATION C1 5956617 d without license from IHS