ISO INTERNATIONAL STANDARD 7346-3 Second edition 1996-06-15 Water quality Determination of the acute lethal toxicity of substances to a freshwater fish [Brachydanio rerio Hamilton-Buchanan (Teleostei, Cyprinidae)J - Part 3: Flow-through method Qualité de I'eau - Détermination de la toxicite aigue létale de substances vis-a-vis d'un poisson d'eau douce [Brachydanio rerio Hamilton-Buchanan (Téléostei, Cyprinidae Partie 3: Methode avec renouvellement continu This material is reproduced from iso documents under International Organization for Standardization (iso) Copyright License number tHS/IcC/1996. Not for resale. No part of these Iso documents may be reproduced in any form, electronic retrieval system or otherwise, except as aliowed in the copyright iaw of the country of use, or with the prior written consent of ISo (Case postale 56, 1211 Geneva 20, Switzerland, Fax +41 22 7341079),IHs ortheiso Licensor'smembers. Reference number ISO 7346-3:1996(E) Copyright International Organization for Standardization No reproduction or networking permitted without license from IHS Not for Resale ISO 7346-3:1996(E) Foreword ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (lsO member bodies). The work of preparing International Standards is normally carried out through Iso which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with sO, also take part in the work. IsO collaborates closely with the International Electrotechnical Commission (lEC)onallmattersofelectrotechnicalstandardization. circulated to the member bodies for voting. Publication as an International a vote. ISO/Tc 147, Water quality, Subcommittee SC 5, Biological methods. This second editioncancelsandreplacesthefirst edition (lS07346-3:1984).whichhasbeentechnicallyrevised. quality Determination of the acute lethal toxicity of substances to a freshwater fish [Brachydanio rerio Hamilton-Buchanan (Teleostei, Cyprinidael Part 1:Static method --- Part 2: Semi-static method Part 3:Flow-through method Annexes A, B and C of this part of ISO 7346 are for information only. ISO1996 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying and microfilm, without permission in writing from the publisher. International OrganizationforStandardization Case Postale 56 · CH-1211 Geneve 20 : Switzerland Printed inSwitzerland i No reproduction or networking permitted without license from IHS Not for Resale C ISO ISO7346-3:1996(E) Introduction The three parts of ISO 7346 describe methods of determining the acute Hamilton-Buchanan) but it must be emphasized that the recommended use of the zebra fish does not preclude the use of other species. The freshwater, marine or brackish water fish, with appropriate modifications of, for example, dilution water quality and the temperature conditions of the test. Within the three parts of ISO 7346, a choice can be made between static, semi-static and flow-through methods. The static test, described in IsO 7346-1, in which the solution is not renewed, has the advantage of requiring simple apparatus, aithough the substances in the test vessel may the water may deteriorate. The flow-through method, described in this part of isO 7346, in which the test solution is replenished continuously. atus. In the semi-static procedure, described in IsO 7346-2, the test sol- utions are renewed every 24 h or 48 h, this method being a compromise between the othertwo. The flow-through method can be used for most types of substances, in- cluding those unstable in water, but the concentrations of the test sub- stance are determined wherever possible. The static method is limited to the study of substances whose tested concentrations remain relatively constant during the